The Myrtle K. Aydelotte papers date from 1934 to 2001 and measure 21 linear feet. The papers are arranged in eight series: Personal, Advising and consulting, Conferences, Employment, Professional associations, Professional correspondence, Research, and Photographs.

The Personal series is devided into nine subseries: activities and interests, biographical, correspondence, education at the University of Minnesota, education at the State University of Iowa (not the University of Iowa), education at Stanford University, education, honors and awards, and gifts. The activities and interests subseries contains information about various associations she was involved with during her time at school, as a nurse, and as a professional teacher. The biographical subseries has autobiographies of Myrtle Aydelotte and her husband William Aydelotte as well as newspaper clippings and rememberances of Aydelotte's time in the army. The correspondence subseries focuses on her personal correspondence with friends and family. The four education subseries contain class notes from her time both as a student and a nursing professor. The honors and awards subseries includes the honors she recieved from 1949 all the way to 2001. The gifts subseries focuses on gifts she revieved and gave to various institutions.

The Advising and consulting series (1950-2001 and undated) consists of correspondence and Aydelotte's notes resulting from her many consulting positions. This series documents Aydelotte's independent consulting visits as well as the advisory and editorial boards on which she served.

The Conferences series (1949-1996 and undated) mainly consists of papers Aydelotte gave at conferences, workshops, and seminars during her career. The correspondence related to the events is also included.

The Employment series (1945 -1996 and undated) is divided into four main subseries: Army, State University of Iowa College of Nursing, University of Iowa Hospitals and Clinics, and Veteran's Administration. These subseries contain Aydelotte's administrative papers, committee materials, and other materials which relate to her four main places of employment.

The Professional associations series (1950-1999 and undated) includes the records pertaining to her involvement in professional organizations. The three most prominently represented here are the American Nurses Association, the National League for Nursing, and Sigma Theta Tau.

The Professional correspondence series (1957-2000 and undated) includes all the correspondence that Aydelotte specifically labeled as professional. Additional correspondence can be found throughout the collection.

The Research series (1951-1998 and undated) contains papers Aydelotte wrote for publication and her notes and drafts from her large research studies. Correspondence with publishers, editors, and co-authors is interfiled with the publications in this series.

The Photographs series (1976-1990 and undated) contains photographs of Aydelotte with other nurses and one undated portrait. Several of the photographs are of early graduating classes of the University of Iowa College of Nursing.